GAG/proteoglycan content per construct; means ± standard deviation. ST, static culture; B, bioreactor culture; and following number represents time after seeding in weeks. C4S, chondroitin-4-sulfate; C6S, chondroitin-6-sulfate; DSPG, dermatan sulfate proteoglycan. The highest levels appear at week 0, whereas there is a general gradual increase and peak in chondroitin sulfate content at 4 weeks, and a general increase in DSPG throughout.