Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jun 20;16:37. doi: 10.1186/s12868-015-0175-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Al-Shamsi et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Spinal cord caspase activity in immunized and non-immunized DA rats. A representative micrograph of sections of the spinal cord from diseased DA rats (a grade 3.5; n = 4) and immunized but resistant AO (b) rats (n = 4) immunostained with polyclonal rabbit anti-caspase-3. Caspase-3 positive cells (arrows) were observed only in DA rats. c A graph of the number of caspase-3 immunoreactive cells in sections of spinal cords. Spinal cord specimens were also incubated in oxygenated KH buffer plus Ac-DEVD-AMC with and without zVAD-fmk. The tissues were then disrupted by homogenization and the supernatants were separated on HPLC and analyzed for AMC peak. di depicts representative runs of dH₂O and substrate injections, dii runs of spinal cord tissue caspase activity of non-immunized DA rat and diii representative runs of spinal cord tissue caspase activity of immunized DA rat at the peak of clinical disease. div AMC peak areas were plotted as a function of days after immunization in DA rats. A peak, indicative of caspase activity is significant only in DA rats at the peak of clinical disease. This is further confirmed in graphs of AMC peak areas as a function of days after immunization (complete lines). Note how caspase activity rises from induction (1), grade 2 disease (2), till peak of clinical disease (3) and recovers (4) to levels similar to that of (1) after recovery. This is not observed in resistant AO rats (dotted lines). Bar 25 μm.