Fig. 1.

B. pseudomallei biofilm formation phenotypes. a Biofilm formation was measured by crystal violet staining assay using static broth cultures of B. pseudomallei UM1 (low producer) and UM6 (high producer) in a 96-well flat-bottomed microtiter plate and in test tubes. Non-pathogenic B. thailandensis ATCC 700388 was used as the reference strain in this study. b Colony morphology of B. thailandensis ATCC 700388, B. pseudomallei UM1 (low producer) and UM6 (high producer) on Ashdown agar plates after 48 h incubation at 37 °C