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. Author manuscript; available in PMC: 2015 Jun 19.
Published in final edited form as: Int J High Throughput Screen. 2011 Mar 31;2:1–13. doi: 10.2147/IJHTS.S17076

Figure 1.

Figure 1

The green fluorescent protein ionizing radiation-induced foci-binding domain (GFP-IBD) construct expressed in cells functions as a reporter for DNA damage. A) The GFP-IBD reporter construct contains GFP fused to residues 1–34 and 1220–1711 of human 53BP1. The Tudor domains are placed 3′ of GFP and a tetracycline inducible promoter. B) The two panels show flow cytometry of MCF7Tet-On GFP-IBD uninduced (control, 2% GFP-positive) and 48 hours after induction with 1 μg/mL doxycycline when 70% of cells scored as GFP-positive. C) After 48 hours of induction with 1 μg/mL doxycycline, MCF7Tet-On GFP-IBD cells were irradiated with a single dose of 6 Gy. Immunofluorescence staining of endogenous 53BP1 shows colocalization with GFP-IBD at all time points.