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. 2015 Jan 28;34(11):1478–1492. doi: 10.15252/embj.201490546

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© 2015 The Authors. Published under the terms of the CC BY NC ND 4.0 license

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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SroC is the effector molecule for GcvB repression

Expression changes of OppA and GcvB upon deletion of gltIJKL locus. WT (JVS-1574), ΔgltIJKL (JVS-5823), ΔgltJKL (JVS-10795), ΔsroC (JVS-5821), ΔgcvBΔsroC (JVS-5822), and ΔgcvB (JVS-0236) strains were grown to early stationary phase (OD₆₀₀ of 2.0) in LB medium.

Expression changes of OppA and GcvB by overexpression of gltI-sroC. ΔgltIJKL strain (JVS-5823) harboring pBAD-ctrl (lane 1), pBAD-gltI (pMM36) derivatives (lanes 2-5), or pBAD-SroC (lane 6) was grown to early stationary phase (OD₆₀₀ of 2.0) in LB medium supplemented with 0.02% l-arabinose.

Data information: Upper two panels: Total protein was analyzed by Western blot to quantify OppA expression. GroEL served as a loading control. Lower three panels: Total RNA was analyzed by Northern blot, and expression of GcvB and SroC was monitored. 5S rRNA served as a loading control. Estimated size from pUC8 marker is indicated on the right. Source data are available online for this figure.