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. 2015 Jan 28;34(11):1478–1492. doi: 10.15252/embj.201490546

Figure 3.

Figure 3

Posttranscriptional regulation of GcvB by SroC
  1. Salmonella ΔsroC (JVS-5821), ΔgcvBΔsroC (JVS-5822), and ΔsroCΔhfq (JVS-9031) strains were transformed with pBAD-ctrl (pKP8-35) or pBAD-SroC (pYC6-4). ΔgcvBΔsroC was cotransformed with pPL-GcvB (pMM03). Each strain was grown to OD600 of 0.5 (0 min) and was further incubated for 10 min in the presence of 0.2% l-arabinose.
  2. Salmonella WT (JVS-1574), ΔgltIJKL (JVS-5823), ΔCA (JVS-10741), and ΔsroC (JVS-5821) strains were grown to OD600 of 2.0 prior to the addition of rifampicin. Total RNA was analyzed by Northern blot to determine decay rates of GcvB. The half-lives were determined from three independent experiments; the standard deviation is indicated.
Source data are available online for this figure.