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. 2015 Jan 28;34(11):1478–1492. doi: 10.15252/embj.201490546

Figure 6.

Figure 6

SroC induces GcvB degradation by a base-pairing mechanism
  1. GcvB-SroC base-pairing regions. The compensatory base pair changes in GcvB and SroC are indicated in red.
  2. Salmonella ΔgcvBΔsroC (JVS-5822) strain was cotransformed with pPL-GcvB and pBAD-SroC derivatives (see Supplementary Table S4). Each transformant was grown to OD600 of 0.5 (0 min) and incubated for another 10 min in the presence of 0.2% l-arabinose. Total RNA was prepared from the cells and subjected to Northern blot analysis. The fold repression of GcvB was determined from three independent replicates; standard deviation is shown.
  3. The chromosomally modified wild-type sroC strain (JVS-10108), sroC** mutant (JVS-10111) and sroC deletion mutant (JVS-5821) were grown to early stationary phase in LB medium (OD600 of 2.0) prior to the addition of rifampicin. Cultures were harvested at the indicated time points after rifampicin treatment. RNA was isolated and analyzed by Northern blot as in Fig3B.
Source data are available online for this figure.