Figure 7.

SroC ensures expression of GcvB targets during growth on casamino acids

• A–D Salmonella strains with oppA (A and C) and gltI (B and D) translational fusions to a sfgfp reporter gene in WT, sroC**, ΔgcvB, and ΔgcvB sroC** background were grown in minimal medium supplemented with 0.4% casamino acids. Fluorescence of bulk culture (A and B) normalized by OD₅₉₅ was measured in 100 μl culture per well on 96-well plate over growth by TECAN plate reader. Fluorescence of 50,000 cells grown to exponential phase (C and D) was monitored by FACS. See also Supplementary Fig S4.