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. 2015 Apr 20;34(11):1523–1537. doi: 10.15252/embj.201490648

Figure 1.

Figure 1

General features of sequenced total RNA during ZGA
  • A Composition of selected libraries produced from total RNA. Shown is the proportion of reads matching rRNA-derived transcripts (rRNA), transcripts produced from repeats identified by RepeatMasker (repeat), annotated mRNAs (mRNA), and other sequences (other).
  • B Hierarchical clustering of 76-nt paired-end datasets from different stages of preimplantation development performed on Spearman correlations between log2 of RPKM values for transcripts.
  • C, D Comparison of MII and 1-cell transcriptomes (C) and 1-cell and 1-cell + DRB transcriptomes (D). The graph was made by plotting expression values for mRNAs (log2[RPKM + 1]) from 76PE sequencing data. Each point represents one transcript whose position indicates its abundance value in indicated stages. RPKM values were increased by adding 1 in order to obtain log2 values above zero.