Table 1. Detection of leptospiral RNA by quantitative PCR in blood samples collected from humans with suspected leptospirosis.
Assay results | Confirmed leptospirosis (N = 22) | Probable leptospirosis (N = 3) | Healthy Subjects (N = 24) |
---|---|---|---|
Number (%) | |||
RNA and DNA PCR+ | 5 (23) | 0 (0) | 0 (0) |
RNA PCR+ alone | 9 (41) | 2 (67) | 0 (0) |
DNA PCR+ alone | 2 (9) | 0 (0) | 0 (0) |
Total RNA PCR+ | 14 (64) | 2 (67) | 0 (0) |
Total DNA PCR+ | 7 (32) | 0 (0) | 0 (0) |