Table 2. Oligonucleotides used for cloning of T. gondii and P. falciparum constructs, confirmation of construct integration and site-specific recombination.
LIC, ligation independent cloning; for, forward; rev, reverse; mut, mutated; ssr, site specific recombination; integr, integration
| Oligo | 5’- 3’ sequence |
|---|---|
| U1-for | GGATAACAGCAGGTAAGTTGGGAACAGGTAAGTTAAT |
| U1-rev | TAACTTACCTGTTCCCAACTTACCTGCTGTTATCCTGCA |
| mutU1-for | GGATAACAGCATGGAACTTGGGAACATGGAACTTAAT |
| mutU1-rev | TAAGTTCCATGTTCCCAAGTTCCATGCTGTTATCCTGCA |
| floxU1-for | GGCTGCAGGATAACAGATAACTTCGTATAGCATACATTATACGAAGTTATATTAATCACCGTTGTGCTCACTTCTCAAATCGACAAAGGAAACACACTTC |
| floxU1-rev | CCGGCGGCCGCAACTTACCTGTTCCCAACTTACCTGATAACTTCGTATAATGTATGCTATACGAAGTTATTCTAGAACTAGTGGATCCCCCTCGGGGGGGCAAGAATTGTG |
| CHC1-LIC-for | TACTTCCAATCCAATTTAATGCGAAGCTCGCCATCTCGATGGGTGGAGAC |
| CHC1-LIC-rev | TCCTCCACTTCCAATTTTAGCAAAGGGAGCGAAAGACTGTCCGAGATTGGC |
| CHC1-integr-for | CGACTCGGGCAACACCCAAC |
| pG152-integr-rev | CAAGCCACAGCGGAACAA |
| pG152-ssr-for | CGATGTTCCAGATTACGCTGCTGAC |
| pG152-ssr-rev | CGAGATCCAATCCAATGCGGCCGC |
| DrpC-LIC-for | TACTTCCAATCCAATTTAATGCCCGAGTCTCTCTCGGAGGAGGCGGGGAAAGG |
| DrpC-LIC-rev | TCCTCCACTTCCAATTTTAGCAGCCCCATTCAACGGTGACGGAAGCCG |
| DrpC-integr-for | GCGCCACTCACGACGAAGC |
| VPS26-LIC-for | TACTTCCAATCCAATTTAATGCAAAGTTCAGGAACACCTCCGGTCGCGCAAGAAA |
| VPS26-LIC-rev | TCCTCCACTTCCAATTTTAGCCCCGATCTTCTTCCTCCACATTGTGAT |
| VPS26-integr-5’-for | CTCCACATCGAGTTCGAGTACGA |
| VPS26-integr-5’-rev | CGTATAATGTATGCTATACGAAGTTATC |
| VPS26-integr-3’-for | GACCCAATCGTACCCTGTACTTCCAATCC |
| VPS26-integr-3’-rev | TCTCACGATCTCACTCGAAA |
| VPS26-ssr-rev | TTTGTGATGCTCGTCAGG |
| DiCre-for | GCCCTAGGATGGGAGTGCAGGTGGAAACC |
| DiCre-rev | CCTTAATTAATCAGTCCCCATCCTCGAGCAGCC |
| Ku80-for | GACGCCGGATTGTAGTGTCT |
| Ku80-rev | TTCCTGATCCCCGTGTATGT |
| HXGPRT-for | GATATATTTATAGCTAGCGAGATATATCGACTCATGCAGCACGAAACCTTGCATTCAAACCCGCCCGCGG |
| HXGPRT-rev | CTTGTACTCACCCACAGTGAGTATCCCACCGCGGTGTCACTGTAGCCTGCCAGAAC |
| Ku80-5’-for | GAGGTACCGATATCGCAGAGAAAACGAGACCAATGCCGTCG |
| Ku80-3’-rev | GACCGCGGGGATTGACCAAATCGACTGGTGTGTGTAC |
| HXGPRT-5’-integr-for | GCGGGCGGGTTTGAATGCAAGGTTTCGTGC |
| HXGPRT-3’-integr-rev | AAGACCACGTTCTTCATGATAGCTGTACAACAGAGTTGGACGACTGATTTAGATCTTG |
| Endo SUB1 FOR_end | GGGCAGGATATGCAGATATAAATAAGGC |
| PbDT3’ R1 | GCTATTTACATGCATGTGCATGC |
| endo SUB1 REV1 | CCTATTAATAATTATAAGCTTGCATGC |
| GFP qPCR-f | CAAGAGCGCCATGCCTGAGGG |
| GFP-qPCR-r | GCTCGATCCTGTTGACGAGGGTG |
| Act1 qPCR-f | CACGAGAGAGGATACGGCTT |
| Act1-qPCR-r | CTCAGCCGCCTTCATTTC |
| DrpC-qPCR-f | CTCCTAAAGGTCTTGGGCTG |
| DrpC-qPCR-r | CGCTTCAAGACACGCTCG |