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. 2015 Jun 19;10(6):e0130593. doi: 10.1371/journal.pone.0130593

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© 2015 Yang et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — The pair-wise variation (V_n/V_n+1) was analyzed to determine the best number of references genes demanded for qRT-PCR data normalization [1]. The value V6/7 is higher than V5/6; this is due to the inclusion of a relative unstable sixth gene. Increasing variation in this ratio corresponds to decreasing expression stability.