Table 2. A summary of ranking for reference gene candidates using five different statistical approaches.
RefFinder | geNorm | NormFider | ΔCt | BestKeeper | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Genes | GM | Genes | SV | Genes | SV | Genes | SV | Genes [r] | Genes SD | ||
RPS8 | 1.19 | RPL14 | 1.035 | RPS8 | 0.792 | RPS8 | 1.45 | RPL14 | 0.860 | HSP70 | 0.88 |
RPL14 | 2.00 | RPS8 | 1.035 | RPL14 | 0.805 | RPL14 | 1.46 | RPL11 | 0.748 | RPS8 | 0.95 |
RPL11 | 3.00 | RPL11 | 1.092 | RPL11 | 0.955 | RPL11 | 1.54 | 18S | 0.700 | RPL11 | 0.97 |
HSP70 | 3.64 | ATPase | 1.167 | ATPase | 1.14 | ATPase | 1.62 | RPS8 | 0.670 | RPL14 | 1.01 |
ATPase | 4.47 | RPS23 | 1.227 | HSP70 | 1.238 | HSP70 | 1.69 | ATPase | 0.376 | ATPase | 1.03 |
RPS23 | 6.26 | 12S | 1.276 | NADH | 1.367 | NADH | 1.77 | EF1A | 0.299 | RPS23 | 1.04 |
12S | 6.74 | HSP70 | 1.432 | 12S | 1.388 | 12S | 1.78 | HSP70 | 0.276 | 12S | 1.04 |
NADH | 6.93 | NADH | 1.52 | RPS23 | 1.394 | RPS23 | 1.78 | NADH | 0.231 | NADH | 1.05 |
EF1A | 9.24 | EF1A | 1.578 | 18S | 1.488 | EF1A | 1.86 | 12S | 0.183 | EF1A | 1.26 |
18S | 9.74 | 18S | 1.639 | EF1A | 1.512 | 18S | 1.89 | RPS23 | 0.001 | 18S | 1.40 |
12 samples were from developmental stage group as input.
Geometric mean (GM); Stability Value (SV); Pearson’s correlation coefficient ([r]); Standard Deviation (SD).