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. 2015 Jun 19;10(6):e0130749. doi: 10.1371/journal.pone.0130749

Fig 5. Histamine stimulated VECs on hydrogel based platforms are capable of promoting platelet adhesion.

Fig 5

(A) Endothelialized peptide-immobilized hydrogel platforms significantly reduce platelet adhesion. Platelet adhesion on hydrogel scaffolds increases with increasing molecular weight PEGDA hydrogel. Platelet adhesion is significantly reduced on VEC-seeded peptide-coated hydrogels (gray bars) when compared to acellular peptide-coated constructs (white bars). * p <0.05. (B) Total adhered platelets onto stable and stimulated VECs cultured on TCPS and hydrogels with immobilized RKR or RGDS. Histamine stimulated VECs (black bars) of all culture condition promoted significantly more platelet adhesion when compared to the non-simulated VECs cultured on the same condition (gray bars). * p < 0.05. Platelet adhesions were significantly lower for the stimulated VECs on RGDS gels than for all other stimulated conditions. # p < 0.05. (B) Dyed platelets adhered onto anchored ultra-large VWF strings (yellow arrows) on the surface of histamine stimulated VECs cultured on a RKR-3.4 kDa hydrogel. Scale bar = 50 μm.