Fig 1. DGGE analysis of the bacterial community in the pig ileal loops with or without attaching and effacing (AE) lesion after inoculation of E. coli O157:H7 strain 86–24.
The bacterial community profiles were generated by targeting 16S rRNA gene from reverse transcribed total intestinal bacterial RNA of the ileal contents. Figures A and B are overlapped top and lower portions of the same DGGE profiles but from different DGGE pictures with different resolutions. The profiles included control ileal loops inoculated with EMEM (control-loops), ileal loops without AE lesion and with AE lesion after E. coli O157:H7 inoculation (ileum-loops AE- and ileum-loops AE+ respectively), and E. coli O157:H7 strain 86–24 grown in vitro before inoculation (In vitro).