Table 2. Gene expression profiles of O157:H7 in pig intestinal loops as determined by qPCR.
| Functional category and gene | Relative fold expression in intestinal loops | ||
|---|---|---|---|
| AE-negative loops | AE-positive loops | Control loops | |
| LEE genes | |||
| tir | 1±0.54 | 18.11±13.5* | 0.16±0.06* |
| eae | 1±0.79 | 50.7±25.53* | 0.13±0.06* |
| ler | 1±0.73 | 4.54±2.38* | 0.08±0.48* |
| grlA | 1±0.60 | 4.08±3.24 | 0.04±0.03* |
| grlR | 1±0.38 | 2.41±1.62 | 0.07±0.03* |
| espD | 1±0.5 | 263.6±166.9* | 0.05±0.01* |
| espA | 1±0.33 | 210.76±91.52* | 0.10±0.01* |
| Toxin and putative toxin genes | |||
| vt2A | 1±0.27 | 2.45±1.74 | 1.84±1.29 |
| vt2B | 1±0.40 | 1.99±1.40 | 0.98±0.6 |
| ehxA | 1±0.71 | 0.66±0.39 | 0.17±0.09 |
| stcE | 1±0.63 | 6.09±3.74* | 0.38±0.34 |
| pagC | 1±0.55 | 1.61±1.00 | 0.26±0.23 |
| ent | 1±0.39 | 4.64±1.48* | 0.29±0.13* |
| Adhesins and putative adhesins | |||
| aidA 15 | 1±0.28 | 9.34±5.03* | 0.14±0.08* |
| aidA 48 | 1±0.20 | 5.56±4.47 | 0.45±0.30 |
| iha | 1±0.13 | 6.95±4.44* | 0.22±0.05* |
| fliC | 1±0.98 | 13.49±5.51* | 3.23±0.24 |
| toxB | 1±0.53 | 2.61±1.12 | 0.25±0.24 |
| eaeH | 1±0.28 | 4.78±3.23* | 0.45±0.30 |
| efa1’-a | 1±0.62 | 2.74±1.06* | 0.37±0.31 |
| efa1’-b | 1±0.29 | 1.56±0.95 | 0.35±0.26 |
| lpf 141 | 1±0.47 | 1.34±0.76 | 0.09±0.06* |
| lpf 154 | 1±0.14 | 0.37±0.20* | 0.11±0.08* |
| fimA (pilin) | 1±0.15 | 0.26±0.18* | 0.45±0.42 |
| hcpA | 1±0.55 | 1.27±1.07 | 2.18±1.13 |
| ompA | 1±0.21 | 9.33±3.28* | 4.81±2.79 |
| Global regulatory genes | |||
| himA | 1±0.43 | 5.49±2.03* | 2.38±2.03 |
| hns | 1±0.60 | 6.50±3.13* | 3.99±1.99 |
| hha | 1±0.54 | 1.74±1.40 | 0.94±0.36 |
| bipA | 1±0.97 | 4.37±1.56* | 1.60±0.85 |
| fis | 1±0.96 | 7.57±4.15* | 5.75±4.58 |
| lrp | 1±0.45 | 2.39±0.79 | 1.14±0.57 |
| phoQ | 1±0.99 | 1.34±0.65 | 0.94±0.68 |
| phoP | 1±0.92 | 1.44±0.53 | 1.09±1.05 |
| hfq | 1±0.30 | 2.36±0.83* | 1.01±0.64 |
| etrA | 1±0.21 | 2.14±1.50 | 0.45±0.26 |
| eivF | 1±0.22 | 0.49±0.24* | 0.39±0.24 |
| QS and its regulated genes | |||
| luxS | 1±0.61 | 3.46±1.38* | 3.3±1.8 |
| qseA | 1±0.81 | 1.09±0.54 | 0.41±0.33 |
| qseB | 1±0.53 | 3.24±2.83 | 1.81±1.57 |
| qseC | 1±0.44 | 2.27±1.25 | 1.48±1.16 |
| qseE | 1±0.37 | 2.41±1.94 | 0.72±0.56 |
| qseF | 1±0.11 | 3.57±2.03* | 1.31±0.86 |
| flhD | 1±0.46 | 4.03±3.02 | 3.76±3.57 |
| kdpE | 1±0.71 | 2.72±1.84 | 2.58±1.34 |
| rcsB | 1±0.63 | 3.02±1.61 | 2.01±1.65 |
| Acid response and stress genes | |||
| gadA | 1±0.22 | 2.40±0.78* | 0.25±0.21* |
| gadC | 1±0.34 | 4.85±4.80 | 0.26±0.26* |
| gadE | 1±0.64 | 1.47±0.71 | 1.02±0.39 |
| gadX | 1±0.30 | 1.76±0.93 | 0.60±0.50 |
| gadW | 1±0.84 | 0.89±0.66 | 1.01±0.71 |
| adiA | 1±0.61 | 2.35±1.6 | 2.3±2.23 |
| ureC | 1±0.59 | 3.86±1.62* | 0.49±0.47 |
| ureD | 1±0.50 | 4.97±2.51* | 1.09±0.84 |
| mnmE | 1±0.30 | 2.04±1.29 | 3.19±1.64 |
| ydeO | 1±0.46 | 1.44±1.07 | 0.7±0.32 |
| evgA | 1±0.73 | 0.39±0.28 | 0.91±0.69 |
| katP | 1±0.25 | 2.80±2.10 | 0.05±0.04* |
| sodA | 1±0.89 | 3.54±2.04 | 2.33±2.20 |
| chuA | 1±0.14 | 8.72±5.52* | 0.77±0.25 |
| terC | 1±0.52 | 7.35±1.79* | 0.37±0.14* |
| terF | 1±0.18 | 8.39±4.95* | 0.47±0.33 |
| rpoA | 1±0.33 | 7.21±2.60* | 1.31±0.49 |
| rpoS | 1±0.40 | 6.35±4.41* | 4.23±2.01 |
| Secreted proteins | |||
| espP | 1±0.72 | 2.52±0.95 | 0.67±0.58 |
| espJ | 1±0.19 | 1.11±0.39 | 0.27±0.23* |
| espFu | 1±0.48 | 1.82±0.81 | 0.74±0.34 |
| nleA | 1±0.38 | 14.5±5.59* | 0.57±0.25 |
| nleB | 1±0.67 | 4.74±2.72* | 0.34±0.3 |
| nleD | 1±0.41 | 1.72±1.11 | 0.04±0.04* |
| Genes with unknown function | |||
| yhbM | 1±0.20 | 2.73±1.97 | 0.78±0.51 |
| Z1006 | 1±0.42 | 2.21±1.28 | 1.03±0.63 |
| Z3276 | 1±0.87 | 1.54±1.24 | 0.57±0.30 |
a, Data are presented as relative fold expression (RFE) and represent the changes in extent of transcription compared to that of the bacteria from AE-negative loops (assigned a value of 1.0).
b, Data are expressed as the means ± SD for RNA extracted in 4–6 biological replicates.
c, Control loops were inoculated with EMEM medium only.
* indicates p<0.05 as compared to bacteria from AE-negative loops.