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. Author manuscript; available in PMC: 2015 Jun 19.
Published in final edited form as: Pain. 2010 Aug 1;151(1):174–183. doi: 10.1016/j.pain.2010.07.003

Fig. 5. Distribution of ETA-R and ETB-R in the skin of sham and CPIP mice.

Fig. 5

Two days post I/R injury, the skin of sham (left panels) and CPIP (right panels) mice was incubated with anti-ETA-R (1:4000, top panels, green) or anti-ETB-R (1:2000, bottom panels, green) antibodies, or stained with hemathoxiline and eosin (H&E). All pictures were taken with a 40 X objective. ETA-R staining was predominantly found in the deeper stratum basalis (s.b.) layer and ETB-R in the medium strati granulosum (s.g.) and spinosum (s.s.) layers. No or poor staining was observed in the external stratum corneum (s.c.) There are no obvious changes in ETA-R or ETB-R staining between sham and CPIP mice.