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. 2015 Jun 19;10(6):e0130585. doi: 10.1371/journal.pone.0130585

Fig 2. Expression and Epigenetic Analysis of TESCs.

Fig 2

(A) Relative expression of pluripotency markers by quantitative real-time RT-PCR analysis at passage 10. All data represent the mean and SEM (n = 3). *P < 0.05. (B) Analysis of DNA methylation profiles. DNA methylation levels in Oct3/4 and Nanog genes were determined by Bio-COBRA in TESCs and ESCs. All data represent the mean and SEM (n = 3). *P < 0.05. (C) Immunostaining of pluripotency markers. DAPI was used to stain DNA. A representative figure is shown. Scale bar, 50 μm.