Table 1. Summary of DNA sequencing, assembly, and annotation of Asian citrus psyllid transcript libraries.
Sequencing (clean reads) | Wb | 21,552,866 |
WbL | 46,865,913 | |
Ny | 32,265,958 | |
NyL | 28,947,167 | |
Total | 129,631,904 | |
Assembly | Total contigs | 45,976 |
Mean length (range) (bp) | 1,107 (150–26,540) | |
% GC (range) | 40.4 (10.4–77.9) | |
Annotation | Total annotated contigs (%) | 17,958 (39%) |
Unique hits | 293,195 | |
1st Best hits | 9,352 | |
Total hits | 700,796 | |
Mean length (range) (bp) | 1,980 (150–26,540) | |
% GC (range) | 44.0 (12.3–77.9) | |
E-value (range) | 7.52E-13 (0–1.0E-13) |
Wb = Whole adult body, Ca. Liberibacter asiaticus (CLas)-uninfected; WbL = Whole adult body, CLas-infected; Ny = nymphal instars, CLas-uninfected; NyL = nymphal instars, CLas-infected. The consensus transcript sequences are expressed as reads per KB per million reads, or RPKM units.