Table 3. RT-PCR results of 10 selected ACP transcripts usedselected subjected to validation by RT-PCR, cloning, and sequencing of RNA isolated from Ca. Liberibacter asiaticus-infected adult psyllids demonstrate the integrity of contigs in the database.
| Transcript ID (length bp) 1 | Predicted Annotation (E-value) 2 | UniProt Id | CLas effect on expression 3 | Primer Sequences 5'-3', forward/reverse | |
|---|---|---|---|---|---|
| Nymph | Adult | ||||
| DcWN_04644 | ABC transporter | E0VTI8_PEDHC | -4.6 | -1.6 | GCGGGATCCATGACCATCCCTCGAAACCAA |
| (1947) | (2E-74) | GCGGGGCCCTCACATAGATTTCTTATATTTAAC | |||
| DcWN_34339 | Sugar transporter 1 | C6KIB0_9HEMI | -1.8 | -4.4 | GCGTCTAGAATGGCGGCAGAGACAAAGAT |
| (1437) | (3E-69) | GCGGGGCCCTTAGGCGGCAGCGACACCT | |||
| DcWN_34586 | Putative ferritin 2 | Q4PLJ6_ANOGL | 1.2 | -1.5 | GCGTCTAGAATGAATTCAGTACTACTGAATC |
| (750) | (8E-23) | Q4PLJ6_ANOGL | GCGGGGCCCTTAGGCGGCAGCGACACCT | ||
| DcWN_18755 | Cathepsin B | A9JSH3_MYZPE | -1.5 | -1.4 | GCGTCTAGAATGTTGAATCGTCTGGTCCTA |
| (453) | (2E-106) | GCGGGGCCCGAGATCATCCGATGACAGA | |||
| DcWN_16937 | Glutathione S-transferase | GST1_BLAGE | -1.1 | 1.9 | GCGTCTAGAATGCCGTCTTACAAGCTGTA |
| (612) | (2E-21) | GCGGGGCCCTTAGACTTCGGATTTGGGGCGCT | |||
| DcWN_13848 | Ras-related protein Rac1 | RAC1_DROME | -2.2 | -2.0 | GCGTCTAGAATGCAAGCCATAAAGTGTGT |
| (589) | (4E-7) | GCGGGGCCCTTACAGGAGCACGCAGCGTTT | |||
| DcWN_02176 | Mitogen-activated protein kinase kinase kinase A | E2AGG6_CAMFO | -2.8 | 1.3 | GGAAGGTTCGGCAAAGTGTA |
| (409) | (0) | CGAAGTCTCCCAGCTTCAAA | |||
| DcWN_05944 | Glycerol-3-phosphate dehydrogenase | GPDM_CAEEL | 1.6 | -2.6 | TGGCCCATTATTGGAAAGAA |
| (389) | (0) | CGAATGTCGTTGATGGACAC | |||
| DcWN_07021 | G protein-coupled receptor kinase 2 | GPRK2_DROME | -3.7 | -1.8 | ACTGTTCAACGATTGCATGG |
| (537) | (0) | CCCAGGTCTGAAATCCTCAC | |||
| DcWN_13699 | Aquaporin AQPcic | AQP_CICVR | -1.2 | 1.4 | CTCGAAGCAGTCACACCAAA |
| (307) | (5E-80) | TGCCCAGTAAACCCAAATGT | |||
1. Size of amplicon deposited in GenBank [GenBank: KF907310-KF907319].
2. Description and annotation of the best hit (E-value -10) from UniProt.
3. Numbers represent fold-change in RPKM values. The fold-change ration was determined using TCW.