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. 2015 Jun 19;10(6):e0129248. doi: 10.1371/journal.pone.0129248

Fig 5. Fusogenic effects of CTI and CTII on lipid bilayers.

Fig 5

Calorimetric curves of large unilamellar liposomes containing: DMPC alone (curves 1 and 13); DPPC alone (curves 2 and 14); DMPC+ 5 mol% CL (curve 3); DPPC+ 5 mol% CL (curve 4); DMPC + 5mol% CL + CTII (curve 5); DPPC + 5 mol% CL + CTII (curve 6); DMPC + 5 mol% CL + CTI (curve 7); DPPC +5mol% CL + CTI (curve 8); a mixture of DMPC + 5 mol% CL and DPPC + 5 mol% CL (curve 9); mixture 9 after sonication (curve 10); mixture 9 + CTII (curve 11); mixture 9 + CTI (curve 12); DMPC + 10 mol% DMPS (curve 15); DPPC + 10 mol% DPPS (curve 16); DMPC + 1 0mol% DMPS + CTII (curve 17); DPPC + 10 mol% DPPS + CTII (curve 18); DMPC + 10 mol% DMPS + CTI (curve 19); DPPC + 10 mol% DPPS + CTI (curve 20); a mixture of DMPC + 10 mol% DMPS and DPPC + 10 mol% DPPS (curve 21); mixture 21 after sonication (curve 22); mixture 21 + CTII (curve 23); mixture 21 + CTI (curve 24). The cytotoxin/lipid molar ratio was set at 0.02 for all experimental conditions. This figure shows representative calorimetric traces from three independent experiments that showed similar results. Each sample (curves 1–21) was measured in triplicate.