Fig 5. Altered expression of Notch pathway components in Oune/+ embryos.

Whole mount in situ hybridization with rat Tbx6 (A, B), Mesp2 (C, D), and Dll1 (E, F) probes was performed using E12.5 +/+ and Oune/+ embryos. Obvious changes of expression patterns and intensity were not observed. Red boxes (A, B) and arrowheads (C, D) indicate Tbx6 expression in tail bud and Mesp2 expression in presomitic mesoderm, respectively. The contiguous expression of Dll1 in the presomitic mesoderm (bars, E, F) appear extended anteriorly in heterozygous mutant embryos. Arrows indicates expression borders between somites and presomitic mesoderm. Note that low levels of Dll1 expression were observed in mutants. Original magnification: 20x (A, B); 90x (C, D); 40x (E-F).