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. 2015 Jun 19;10(6):e0130231. doi: 10.1371/journal.pone.0130231

Fig 6. Electrophoretic mobility shift assay (EMSA) of Tbx6 and Tbx6 Oune.

Fig 6

(A) The Tbx6 Oune allele did not influence the DNA binding ability of the T-box binding consensus sequences. Tbx6 and Tbx6 Oune (Tbx6 mut) showed no difference in binding ability to the Tbind probe, two T-box gene binding sites, and to the Tbind-half probe, a single binding site, judged from intensity of shifted bands. Free binding probes were shown at the bottom. (B) DNA binding ability of mutant Tbx6 was not changed. The Tbind probe concentration was diluted to one sixteenth, but no difference was detected between Tbx6-wt and mut. mTbx6 and rTbx6 represents mouse and rat Tbx6, respectively.