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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Am J Transplant. 2015 Feb 27;15(7):1768–1781. doi: 10.1111/ajt.13189

Figure 6. Elafin promotes HUVEC tube formation, migration and wound healing.

Figure 6

(A) Western Blot analysis showing that elafin dose-dependently induces ERK1/2 phosphorylation. PMA was used as a positive control for ERK1/2 phosphorylation, the ERK1/2 inhibitor, PD098059, was used at a concentration of 10μM. (B–S) Elafin dose-dependently promotes HUVEC tube formation (B–G) as measured by the Matrigel assay, HUVEC migration as measured by the Boyden Chamber assay (H–M), and HUVEC wound healing assay (N–S). VEGF was used as a positive control. (T–W) Quantification of total tube length (T), number of branch points per field (U), number of migrated cells in Boyden Chamber assay (V) and number of migrated cells in wound healing assay (W). (n=4–6 per group). Scale bar, 300μm (B–S). Data are shown as mean±SEM. Student’s t test (T–V) or 1-way ANOVA with Tukey’s post test (W). *P<0.05; NS, not significant.