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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Environ Microbiol. 2015 Jan 27;17(6):1977–1990. doi: 10.1111/1462-2920.12673

Figure 2. Fe(III) reduction by D. reducens cell suspensions grown on sulfate reduction.

Figure 2

Washed cell suspensions of D. reducens grown with 28 mM sulfate and 20 mM lactate were tested for iron reduction capability and shown to express the proteome necessary for immediate dissimilatory reduction of Fe(III)-NTA. Cells killed by boiling and an abiotic sulfide control determined by the concentration of sulfide measured following cell washing (0.25 mM) demonstrated lack of iron reduction capability. Cell concentration was 3x109 cells/mL, equal to a protein concentration of ~0.9 mg/mL of protein assuming 60% protein per cell dry mass. Error bars display standard error duplicate reactions.