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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Environ Microbiol. 2015 Jan 27;17(6):1977–1990. doi: 10.1111/1462-2920.12673

Figure 4. Identification of iron reduction activity in the insoluble proteome of D. reducens.

Figure 4

Protein concentration (determined by absorbance at 280nm and presented as mAU), is represented by the black chromatogram, while the gray line presents an overlay of iron reduction activity (μmol Fe(II) formed/minute). 3.a. SAX separation of insoluble protein fraction led to the recovery of a dominant peak at 31′. Further separation with native gel electrophoresis recovered an active iron reductase band (visualized as a pink band at ~244 kDa, designated by gray arrow). SAX= strong anion exchange chromatography. SEC= size exclusion chromatography.