Skip to main content
. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Environ Microbiol. 2015 Jan 27;17(6):1977–1990. doi: 10.1111/1462-2920.12673

Figure 7. Cr(VI) reduction by Dred_2421 and Dred_1685–1686 complex.

Figure 7

The disappearance of Cr(VI) over time is monitored based on the diphenylcarbazide method. In order to reduce the effects of abiotic reduction by NADH, only 1mM NADH was added at time zero. After an additional 1mM NADH was added at 80 minutes, complete Cr(VI) reduction is observed for both Dred_2421 and the Dred_1685–1686 complex. Individual Dred_1685 and Dred_1686 proteins resembled the no protein control. Reactions contained 2 μM purified proteins.