Fig 6. AKT and GSK knockdown inhibits R. microplus hatching rate.

Following electroporation with AKT (A) or GSK (B) dsRNA, de-waxed eggs at the developmental stages indicated were kept at 28° C and 80% relative humidity until hatching. Immediately after hatching, larvae were frozen at -20° C and then subsequently counted as described. Percentage values refer to the number of larvae relative to total number of eggs (in 150 mg aliquots, in replicates). Statistical analysis was carried out using the Student t test (p<0.05), (Triplicate; n = 3).