Fig. 3.

Siglec-9 relocalized in lipid rafts following PGN stimulation. a, b Wild-type or mutated Siglec-9-expressing RAW264 cells were incubated with or without 50 μg/ml of PGN for 10 min, then lysed in lysis buffer, and separated by a sucrose density gradient. Pooled lipid raft fraction was analyzed after concentration (a), while pooled non-raft fractions were analyzed without concentration (b). Data are representative of 2–5 independent experiments