Fig. 4.

Time–course or dose-dependent changes in the AHR-mediated transcriptional activity in stably transfected HepG2 cells after treatment with 3MC. The cells were treated with 3MC (2.5 μM) or with 0.025 % DMSO (control) for the indicated times (a) or were treated with 3MC at the indicated concentrations or 0.1 % DMSO (control) for 24 h (b), and total cell lysates were used for the luciferase assay. Luciferase activity was measured as described in “Materials and Methods”, and the data are represented as a ratio relative to the corresponding controls. Each value is the mean ± SD (n = 3). Time-and dose-dependent effects were analyzed using Tukey multiple comparison test with one-way ANOVA. Points not sharing a common letter are significantly different (p < 0.05)