Fig. 6.

Dose-dependent changes in the mRNA level of AHR-target genes in the stably transfected HepG2 cells after treatment with 3MC. The cells were treated with 3MC at the indicated concentrations or 0.1 % DMSO (control) for 24 h. Total RNA from the cells was used for real-time RT-PCR analysis, and mRNA expression levels of CYP1A1 (treated with 0–10 μM 3MC) (a), CYP1A1 (treated with 0–1 μM 3MC) (b), UGT1A1 (c), and ABCG2 (d) were evaluated. The mRNA levels of AHR-target genes were normalized to those of β-actin mRNA and represented as a ratio relative to the corresponding controls. Each value is the mean ± SD (n = 3). Dose-dependent effects were analyzed using Tukey multiple comparison test with one- way ANOVA. Points not sharing a common letter are significantly different (p < 0.05)