Fig 2. Up-regulated p-IRE1α protein expression level and spliced XBP1 gene expression level in the different cataract lens groups RNA and protein were extracted from human lens specimens.

Western blotting was performed to detect the protein expression of p-IRE1α (Fig 2A, Fig 2B), and β-actin was used as the internal control; real-time PCR was performed to detect the relative gene expression levels of spliced XBP1 in each group (Fig 2C), and 18S rRNA was used as the internal control gene (mean ± SD, n = 3). *P < 0.05; **P < 0.001.