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. 2015 Jun 19;10(6):e0130705. doi: 10.1371/journal.pone.0130705

Fig 3. Up-regulated p-eIF2α protein expression level and ATF4 gene expression level in the different cataract lens groups.

Fig 3

RNA and protein were extracted from human lens specimens. Western blotting was performed to detect the protein expression of p-eIF2α (Fig 3A and 3B), and β-actin was used as the internal control; real-time PCR was performed to detect the relative gene expression level of ATF4 in each group (Fig 3C), 18S rRNA was used as the internal control gene (mean ± SD, n = 3). *P < 0.05; **P < 0.001.