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. 2015 Jun 19;10(6):e0130705. doi: 10.1371/journal.pone.0130705

Fig 4. Up-regulated cleaved ATF6 protein and gene expression levels in the different cataract lens groups.

Fig 4

RNA and protein were extracted from human lens specimens. Western blotting was performed to detect the protein expression of cleaved ATF6 (Fig 4A and 4B), and β-actin was used as the internal control; real-time PCR was performed to detect the relative gene expression level of ATF6 in each group (Fig 4C), and 18S rRNA was used as the internal control gene (mean ± SD, n = 3). *P < 0.05; **P < 0.001.