Fig. 1.

Effects of constitutive Bcl-2 expression on the morphology of MCF10ATG3B cells. a Western blot analysis of the cell lysates from Bcl-2-transfected cells (Bcl-2-1 and −3) and control cells transfected with empty expression vector (CMV-5 and −6) using an anti-Bcl-2 specific antibody and an anti-actin antibody to ensure an equal loading. b BCl-2-1 and CMV-5 cells were cultured in different concentrations of serum as indicated for three days, or cultured in medium with or without hydrocordisone for two days, or treated with different concentrations of TNF for two days. Cell survival assays were performed as described in Methods