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. 2015 Jun 20;15:476. doi: 10.1186/s12885-015-1485-5

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© An et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Constitutive Bcl-2 expression induces a migratory and invasive phenotype. a The motility behavior of control cells and Bcl-2 transfected cells was analyzed in an in vitro wound-healing assay. b Statistical significance (p < 0.01) exhibited for the Bcl-2 transfected cells with stronger migratory capability (N = 3; mean = 65.33; SE = 3.51) than the control ones (N = 3; mean = 11; SE = 2.0).. Data showed the counted cell numbers, and represented as mean ± s.e.m. c The invasive features of the control and Bcl-2 expressing cells were analyzed in an invasion assay on matrigel in a BioCoat Matrigel Invasion Chamber. The cells adhered to the lower surface of the filters were fixed and stained with a Diff-Quik Stain Set, photographed under a light microscope. d Statistical significance (p < 0.01) exhibited for the Bcl-2 transfected cells with stronger invasive potential (N = 3; mean = 57.67; SE = 4.51) than the control ones (N = 3; mean = 4.67; SE = 1.53)