Fig. 4.

Immunohistochemistry of Intrarenal RAS Components in Polycystic Kidneys. Immunohistochemistry was performed to evaluate the expression levels of intrarenal RAS components in the polycystic kidneys (PKD-CKD and PKD-ESRD) compared to normal control kidneys. AGT was highly expressed in the proximal tubules and cyst-lining epithelial cells in polycystic kidneys whereas normal kidney did not express AGT in either glomeruli or tubules. Other intrarenal RAS components (AngII, Ang-(1-7), ACE2, and chymase) were highly expressed in polycystic kidneys compared to the normal kidney. However, the expression level of ACE was lower in the polycystic kidneys compared to the normal control. Magnification x400. ACE, angiotensin converting enzyme; ACE2, angiotensin converting enzyme 2; AGT, angiotensinogen; AngII, angiotensin II; Ang-(1-7), Angiotensin (1-7); CKD, chronic kidney disease; ESRD, end-stage renal disease; PKD, polycystic kidney disease; RAS, renin-angiotensin system