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Representative sections of the proximal tibia cancellous bone from the control and caffeine-fed groups. The tissues were stained with alcian blue; collagen is blue. (A–C) Sections including primary and secondary Sn at 50-fold magnification, and (D–F) sections showing a part of the secondary Sn at 100-fold magnification. (A, D) Control, (B, E) CF1, and (C, F) CF2. CT, control; CF1, caffeine 120 mg kg−1 day−1; CF2, caffeine 180 mg kg−1 day−1. Scale bar: 200 μm.
