Table 3.
Effects of caffeine on histomorphometric parameters of the cancellous bone analyzed using the proximal tibia
| CT (N = 17) | CF1 (N = 16) | CF2 (N = 14) | |
|---|---|---|---|
| Height (mm) | |||
| Whole Sn | 3.30 ± 0.57 | 2.77 ± 0.40** | 2.50 ± 0.51** |
| 1° Sn | 0.65 ± 0.07 | 0.52 ± 0.07*** | 0.46 ± 0.08*** |
| 2° Sn | 2.65 ± 0.56 | 2.25 ± 0.40* | 2.03 ± 0.54** |
| % to the whole | |||
| 1° Sn | 20.87 ± 3.88 | 19.61 ± 4.10 | 20.05 ± 6.34 |
| 2° Sn | 79.13 ± 3.88 | 80.39 ± 4.10 | 79.95 ± 6.34 |
| 2° Sn | |||
| B.Ar (mm2) | 0.24 ± 0.05 | 0.18 ± 0.02** | 0.19 ± 0.03** |
| Bone perimeter (mm) | 13.52 ± 2.35 | 11.85 ± 1.60 | 12.53 ± 2.48 |
| B.Ar/T.Ar (%) | 19.66 ± 4.04 | 14.60 ± 1.59*** | 15.16 ± 2.70** |
| Tb.Wi (μm) | 35.96 ± 4.74 | 30.55 ± 2.43** | 30.11 ± 3.10** |
| Tb.N (mm−1) | 5.53 ± 1.01 | 4.85 ± 0.69 | 5.08 ± 1.01 |
| Tb.Sp (μm) | 154.30 ± 30.57 | 183.02 ± 31.98* | 178.99 ± 43.33 |
B.Ar./T.Ar., trabecular bone volume; CF1, caffeine 120 mg kg−1 day−1; CF2, caffeine 180 mg kg−1 day; CT, control; Sn, spongiosa; Tb.N, trabecular number; Tb.Sp, trabecular separation; Tb.Wi, trabecular width.
Values are expressed as mean ± SD. The height measurements represent the longitudinal dimension of the structure parallel to the long axis of the bone. Whole fields of vision on four consecutive alcian blue-stained sections of each tibia per animal were evaluated for the analysis of the secondary Sn at 100-fold magnification.
*
P < 0.05,
**
P < 0.01,
***
P < 0.001 vs. CT.