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. 2015 Mar 8;4(3):409–422. doi: 10.1002/mbo3.248

Figure 5.

Figure 5

Zinc tolerance assay of yeast expressing AhPDF1.1b::KDEL. (A) Drop tests were performed with control yeast cells expressing the pYX212 empty vector (EV), or the native AhPDF1.1b (AhPDF1.1b) or a recombinant AhPDF1.1b defensin fused with the KDEL retention signal at its C-terminal end (AhPDF1.1b::KDEL). An overnight yeast culture was diluted to the A600nm levels indicated and 10 μL were spotted on YNB medium supplemented or not with 30 mmol/L ZnSO4. (B) Growth curves in liquid cultures contaminated by 20 mmol/L ZnSO4, of control yeast cells expressing the pYX212 empty vector (EV), or the native AhPDF1.1b (AhPDF1.1b) or a recombinant AhPDF1.1b defensin fused with the KDEL retention signal at its C-terminal end (AhPDF1.1b::KDEL). The growth was followed by A600nm measurement.