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Co-immunoprecipitation of L1 ORF1 and A3 proteins. 293T cells were cotransfected with plasmids encoding L1 ORF1 (pcDNA3.1ORF1.V5) and A3A (pKA3A.HA), A3B (pKA3B.HA), mA3 (pFLAG.mA3) proteins. At 48 h after transfection cells were lysed and immunoprecipitation with anti-HA or anti-FLAG antibodies. Immunobloting was performed with anti-V5 antibodies. Addition of RNase A during the immunoprecipitation is indicated.
