Figure 2.

A: Representative signal pattern observed when the PTEN gene is intact and two copies ofthe gene and allchromosome 10 probes are present as two copies. B: Nuclear signal pattern observed for PTEN hemizygous deletions. C: Homozygous PTEN deletion (both copies lost). D: Scoring schemaused to classify FISH signals present in interphase nuclei based on the colored labels used for each probe. The schema only shows examples with simple interstitial deletions affecting the PTEN gene (yellow spot loss) only. In some tumors, larger deletions extending from WAPAL (green) to FAS (blue) were detected. In addition, five tumors with loss arising as a monsomy of chromosome 10 were detected.