Skip to main content
. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Tuberculosis (Edinb). 2015 May 14;95(4):476–489. doi: 10.1016/j.tube.2015.04.002

Figure 7. The Rv3631 and Rv3779 GalN-deficient mutants stimulate more production of TLR2-mediated NF-κB than their WT Mtb parents and complemented mutant strains.

Figure 7

HEK-Blue™ hTLR2 cells were incubated either alone, or with the TLR2 agonist PAM3CSK4 or with the indicated Mtb and M. smegmatis strains at an MOI of 3. Cells and bacteria were cultured in an alkaline phosphatase detection medium for 6 hours and the change in color of the medium due to secreted SEAP was measured at 650 nm on a plate-reading spectrophotometer. Results are shown as the mean + SD of triplicate cultures. P values are indicated on the figure.