Fig. 2.
Effect of estradiol (E2) treatment on on steroidogenic enzymes gene expression in the ARC-ME of ovariectomized (OVX) rhesus macaques. Each bar represents the mean (± sem) mRNA levels of four animals, normalized to the arithmetic mean of two housekeeping genes, ALG9 and RPL13A. In the young animals (a) there was no significant effect of E2 on any of the mRNA levels examined. In the old animals (b), HSD17B5 mRNA levels were significantly higher in the OVX + E2 relative to OVX group. * P < 0.05, (Paired Student’s t-test).