Table 3.
Gene Expression in the Arcuate-Median Eminence of Rheus Macaques During Reproductive Aging
| Gene Symbol | Gene Name | Young Intact | Old OVX | Old OVX + E2 |
|---|---|---|---|---|
| Sex-steroid Receptors | ||||
| AR | androgen receptor | 1.00 ± 0.13 | 0.91 ± 0.10 | 1.02 ± 0.07 |
| ESR1 | estrogen receptor 1 | 1.00 ± 0.11 | 1.19 ± 0.06 | 0.90 ± 0.07 |
| ESR2 | estrogen receptor 2 | 1.00 ± 0.18 | 1.22 ± 0.13 | 1.08 ± 0.14 |
| PGR | progesterone receptor | 1.00 ± 0.10 ** | 0.33 ± 0.13 ** | 1.82 ± 0.14 |
| Reproduction | ||||
| GNRH1 | gonadotropin-releasing hormone 1 | 1.00 ± 0.17 | 1.09 ± 0.33 | 0.89 ± 0.20 |
| GNRH2 | gonadotropin-releasing hormone 2 | 1.00 ± 0.15 | 0.59 ± 0.07 * | 3.31 ± 1.01 |
| KISS1 | KiSS-1 metastasis-suppressor | 1.00 ± 0.32 ** | 11.72 ± 2.20 ** | 0.25 ± 0.08 |
| KISS1R | KISS1 receptor | 1.00 ± 0.11 * | 1.99 ± 0.15 | 2.18 ± 0.38 |
| PDYN | prodynorphin | 1.00 ± 0.15 | 0.89 ± 0.06 | 1.19 ± 0.07 |
| TAC3 | tachykinin 3 | 1.00 ± 0.09 ** | 3.21 ± 0.64 ** | 0.49 ± 0.05 |
| Metabolism | ||||
| AGRP | agouti-related protein homolog | 1.00 ± 0.25 | 1.48 ± 0.24 | 1.00 ± 0.07 |
| INSR | insulin receptor | 1.00 ± 0.16 | 1.27 ± 0.25 | 1.12 ± 0.07 |
| LEPR | leptin receptor | 1.00 ± 0.09 | 1.23 ± 0.17 | 1.12 ± 0.27 |
| MC2R | melanocortin 2 receptor (adrenocorticotropic hormone) | 1.00 ± 0.32 | 0.37 ± 0.11 | 0.69 ± 0.43 |
| MC4R | melanocortin 4 receptor | 1.00 ± 0.29 | 0.62 ± 0.07 | 0.71 ± 0.11 |
| MCHR1 | melanin-concentrating hormone receptor 1 | 1.00 ± 0.05 | 1.22 ± 0.17 | 1.71 ± 0.34 |
| NPY | neuropeptide Y | 1.00 ± 0.53 | 0.81 ± 0.40 | 0.40 ± 0.05 |
| NPY1R | neuropeptide Y receptor Y1 | 1.00 ± 0.19 | 1.08 ± 0.28 | 1.05 ± 0.16 |
| NPY2R | neuropeptide Y receptor Y2 | 1.00 ± 0.15 * | 1.53 ± 0.12 * | 1.04 ± 0.12 |
| NPY5R | neuropeptide Y receptor Y5 | 1.00 ± 0.12 | 1.05 ± 0.17 | 1.31 ± 0.17 |
| POMC | proopiomelanocortin | 1.00 ± 0.19 | 1.17 ± 0.27 | 0.83 ± 0.11 |
| Neurotransmission | ||||
| ACHE | acetylcholinesterase | 1.00 ± 0.10 | 1.17 ± 0.04 | 1.13 ± 0.10 |
| ADRA1A | adrenergic, alpha-1A, receptor | 1.00 ± 0.14 | 0.80 ± 0.03 | 0.73 ± 0.21 |
| DRD1 | dopamine receptor D1 | 1.00 ± 0.10 | 0.89 ± 0.08 | 0.82 ± 0.09 |
| DRD2 | dopamine receptor D2 | 1.00 ± 0.21 | 0.82 ± 0.11 | 1.02 ± 0.22 |
| SLC18A2 | solute carrier family 18, member 2 (vesicular monoamine) | 1.00 ± 0.22 | 0.88 ± 0.08 | 1.07 ± 0.26 |
| SLC6A3 | solute carrier family 6, member 3 (dopamine transporter) | 1.00 ± 0.34 | 0.62 ± 0.06 ** | 4.52 ± 0.74 |
| SLC6A4 | member 3, solute carrier family 6, member 4 (serotonin transporter) | 1.00 ± 0.57 | 0.67 ± 0.62 | 0.55 ± 0.25 |
| GABBR1 | gamma-aminobutyric acid B receptor, 1 | 1.00 ± 0.06 | 0.99 ± 0.02 | 1.08 ± 0.10 |
| GABBR2 | gamma-aminobutyric acid B receptor, 2 | 1.00 ± 0.27 | 0.69 ± 0.04 | 0.77 ± 0.05 |
| GABRA1 | gamma-aminobutyric acid A receptor, alpha 1 | 1.00 ± 0.23 | 0.75 ± 0.03 | 1.01 ± 0.07 |
| GABRA5 | gamma-aminobutyric acid A receptor, alpha 5 | 1.00 ± 0.12 | 0.82 ± 0.06 | 0.87 ± 0.16 |
| HTR1A | 5-hydroxytryptamine (serotonin) receptor 1A | 1.00 ± 0.14 | 0.71 ± 0.06 | 0.86 ± 0.15 |
| HTR2A | 5-hydroxytryptamine (serotonin) receptor 2A | 1.00 ± 0.04 | 0.81 ± 0.12 | 0.63 ± 0.07 |
| HTR2C | 5-hydroxytryptamine (serotonin) receptor 2C | 1.00 ± 0.12 | 1.35 ± 0.15 | 1.20 ± 0.06 |
RT2 Profiler™ PCR Arrays were used to profile gene expression in young intact, old ovariectomized (OVX), and old OVX estrogen-treated (OVX+E2) females. The values (mean ± sem) are expressed relative to the arithmetic mean of ALG9, GAPDH, and RPL13A, and normalized against the Young Intact group.
*
P < 0.05,
**
P < 0.01 (relative to the Old OVX group; ANOVA followed by the Dunnett test).