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. Author manuscript; available in PMC: 2016 Jun 19.
Published in final edited form as: Circ Res. 2015 Apr 22;117(1):e1–e11. doi: 10.1161/CIRCRESAHA.117.305844

Figure 3. Ectopic apoE expression enhances miR-146a expression in macrophages and monocytes.

Figure 3

A, BMDM prepared from Apoe−/− mice were transfected with an empty vector (Vector) or increasing amounts ranging from 5 to 125 ng/ml of an apoE expression vector using Lipofectamine 2000. After 48 hrs, cells were analyzed for apoE mRNA levels. The results were compared to WT apoE mRNA levels set arbitrarily to 1. B, Apoe−/− BMDMs were transfected as described in (A) and were examined for miR-146a levels. MiR-146a expression in Apoe−/− BMDM receiving control vector were set arbitrarily to 1. C, miR-146a expression in Apoe−/− Ly-6Chigh monocytes transfected with control empty vector or apoE expression vector (125 ng/ml). D, WT monocytes were transfected with a control siRNA (siCtrl) (Ctrl siRNA) or an apoE siRNA (ApoE siRNA) using Lipofectamine RNAiMAX. Cells were analyzed for miR-146a expression 48 hrs after transfection. The results of control groups were set arbitrarily to 1. Similar results were obtained in 3 separate experiments using 4 mice per group. Data are shown as the means ± SEM. *, P<0.05; **, P<0.01.