Figure 5. Hyperlipidemic HypoE mice expressing reduced apoE levels display enhanced miR-146a in macrophages and monocytes that suppress their cellular activation.
MiR-146a expression in (A) peritoneal macrophages and (D) bone marrow Ly-6Chigh monocytes isolated from Apoe−/−Ldlr−/− and Apoeh/hLdlr−/− (HypoE) mice (n=6). (B) The number of macrophages in the aortas of Apoe−/−Ldlr−/− and Apoeh/hLdlr−/− mice (n=4). (C) TNF-α expression in peritoneal macrophages isolated from Apoe−/−Ldlr−/− and Apoeh/hLdlr−/− mice (n=6). The number of (E) blood Ly-6Chigh monocytes and (F) splenic TNF-α-producing Ly-6Chigh monocytes in Apoe−/−Ldlr−/− and Apoeh/hLdlr−/− mice (n=9). The results of Apoe−/−Ldlr−/− mice were set arbitrarily to 1 for A, C and D. Data are shown as the means ± SEM. *, P<0.05; **, P<0.01.