(A and B) Average distribution of H3K4me3 (A) and H3K4me1 (B) ChIP-seq signal for Pol II-bound unidirectional promoters (black; N=2,240) and bidirectional promoters (red; N=8,730) in 50 bp bins centered on sense TSSs.
(C) Shown are H3K27Ac and p300 ChIP-seq reads in the region from the sense TSS to −1 kb at unidirectional (black) and bidirectional (orange) genes. Asterisks signify P<0.0001.
(D) H3K4me1 ChIP-seq signal at bidirectional promoters in a 2 kb window centered on anti-sense TSSs. Bidirectional promoters are ranked by decreasing H3K4me1 signal in the region from −1 kb to the observed anti-sense TSS, and divided into the top quartile of H3K4me1 (red) and all other quartiles of H3K4me1 signal (green), as shown at right.
(E) H3K27ac, p300 and H3K4me3 ChIP-seq reads in the region from the sense TSS to −1 kb at genes from the top quartile of upstream H3K4me1 signal (red) and all other quartiles (green), as shown in (D). Asterisks signify P<0.0001.
(F) Elongated nascent RNA (sense TSS to +250 bp) and Start-RNA reads (±100 bp around sense TSS) at H3K4me1 quartiles as shown in (E). Asterisks signify P<0.0001.
(G) Enriched GO biological processes among bidirectional promoters with highest levels of H3K4me1 (top quartile).
See also Figure S6.