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. Author manuscript; available in PMC: 2016 Jun 18.
Published in final edited form as: Mol Cell. 2015 May 28;58(6):1101–1112. doi: 10.1016/j.molcel.2015.04.006

Figure 6. Enhancer-like Chromatin Signature Around Anti-sense TSSs.

Figure 6

(A and B) Average distribution of H3K4me3 (A) and H3K4me1 (B) ChIP-seq signal for Pol II-bound unidirectional promoters (black; N=2,240) and bidirectional promoters (red; N=8,730) in 50 bp bins centered on sense TSSs.

(C) Shown are H3K27Ac and p300 ChIP-seq reads in the region from the sense TSS to −1 kb at unidirectional (black) and bidirectional (orange) genes. Asterisks signify P<0.0001.

(D) H3K4me1 ChIP-seq signal at bidirectional promoters in a 2 kb window centered on anti-sense TSSs. Bidirectional promoters are ranked by decreasing H3K4me1 signal in the region from −1 kb to the observed anti-sense TSS, and divided into the top quartile of H3K4me1 (red) and all other quartiles of H3K4me1 signal (green), as shown at right.

(E) H3K27ac, p300 and H3K4me3 ChIP-seq reads in the region from the sense TSS to −1 kb at genes from the top quartile of upstream H3K4me1 signal (red) and all other quartiles (green), as shown in (D). Asterisks signify P<0.0001.

(F) Elongated nascent RNA (sense TSS to +250 bp) and Start-RNA reads (±100 bp around sense TSS) at H3K4me1 quartiles as shown in (E). Asterisks signify P<0.0001.

(G) Enriched GO biological processes among bidirectional promoters with highest levels of H3K4me1 (top quartile).

See also Figure S6.