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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Mol Neurobiol. 2014 Dec 20;53(2):867–878. doi: 10.1007/s12035-014-9056-3

Figure 6. Cofilin knock-down increases neuronal viability and reduces cofilin mitochondrial translocation and caspase 3 cleavage.

Figure 6

Primary cultures of cortical neurons at DIV 4 were transfected with cofilin1 siRNA for 48 h and then subjected to 1 h of OGD and 24 h of reperfusion. At DIV 7, neurons were harvested and analyzed for MTT assay and also fractionated to get cytosolic and mitochondrial fractions. A. Cofilin knock-down with siRNA (25 and 50nM) and subjecting to OGD/R rescued neuronal death. B. Representative blot shows a decrease in mitochondrial cofilin levels in cofilin knock-down neurons as compared to control. D. Representative blot of cytosolic fractions shows a decrease in cleaved caspase 3 in cofilin knock-down neurons as compared to the control. C and E. Graphs show densitometric analysis of cofilin normalized to α-tubulin in cytosolic fraction and COXIV in mitochondrial fraction used as a loading control. Data are expressed as mean±SEM of three independent experiments, where p<0.05 was considered significant. *vs control (scrambled siRNA).