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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: J Immunol. 2015 May 15;195(1):116–125. doi: 10.4049/jimmunol.1402473

Figure 2.

Figure 2

CD8+ T cell exhaustion is exacerbated in LCMV clone-13 infected septic mice. Inhibitory molecule expression of splenocytes on day 8 post-LCMV clone-13 infection. (A) Summary data of geometric mean fluorescence intensity (gMFI) of PD-1+, Lag-3+, and 2B4+ expression on tetramer+ GP276-specific CD8+ T cells. Ex vivo GP276-peptide stimulation and staining of splenocytes on day 8 post-LCMV clone-13 infection. Summary data of gMFI of (B) IFNγ+ and (C) TNFα+ expression. Summary data of the percentage of (D) IFNγ and (E) TNFα producing CD8+ T cells of tetramer+ GP276-specific CD8+ T cells. (F) Viral titers were determined from the kidney, liver, and serum at day 8 post-LCMV clone-13 infection. Dots represent individual mice. Data analyzed by two-tailed, unpaired Student t test (4–5 mice/group). Data are representative of two to three independent and similar experiments. * p < 0.05, ** p < 0.01, *** p < 0.0001.